Antiadhesive activity of hydroethanolic extract from bean pods of Phaseolus vulgaris (common bean) against uropathogenic E. coli and permeability of its constituents through Caco-2 cells monolayer

Phaseaoli pericarpium (bean pods) is a pharmacopeial plant material traditionally used as diuretic and antidiabetic agents. Diuretic activity of pod extracts was reported first in 1608. Since then Phaseoli tea figures many textbooks medicinal by patients. Despite the traditional use from Phaseolium vulgaris pericarp, limited information available on bioactivity, chemical composition, bioavailability such preparations. The following study aimed to investigate phytochemical vitro permeability selected extract's constituents over Caco-2 permeation system, potential antivirulence against uropathogenic Escherichia coli hydroalcoholic extract (PPX) support its remedy urinary tract infections. composition PPX [ethanol:water 7:3 (v/v)] investigated using UHPLC-DAD-MSn subsequent dereplication. compounds present evaluated monolayer system. influence E. (UPEC) strain NU14 proliferation bacterial adhesion T24 epithelial cells determined turbidimetric assay flow cytometry, respectively. mitochondrial host monitored MTT assay. LC-MSn investigation dereplication, indicated be dominated variety flavonoids, with rutin major compound, soyasaponin derivatives. Rutin, soyasaponins fatty acids were shown permeate indicating oral intake. did not viability after 1.5h incubation at 2 mg/mL UPEC. significantly reduced UPEC human bladder concentration-dependent manner (0.5–2 mg/mL). Detailed investigations different protocols that seems interact cells, which subsequently leads recognition cell membrane. characterised presence flavonoids (e.g. rutin) saponins, might bioavailable application, experiments. Rutin some saponins can considered potentially inhibition justifies prevention treatment